Low density lipoprotein (LDL) cholesterol is converted to 27-hydroxycholesterol in human fibroblasts. Evidence that 27-hydroxycholesterol can be an important …
M Axelson, O Larsson - Journal of Biological Chemistry, 1995 - ASBMB
The formation of oxysterols in cultured human fibroblasts and their physiological roles as
intracellular regulators of cholesterol production have been investigated. In the presence of
low density lipoproteins (LDL) normal fibroblasts converted LDL cholesterol to the oxysterol
27-hydroxycholesterol in quantities apparently sufficient to down-regulate 3-hydroxy-3-
methylglutaryl coenzyme A (HMG-CoA) reductase. The production of 27-hydroxycholesterol
started 3-8 h after the addition of LDL to the incubation medium, and during this time the …
intracellular regulators of cholesterol production have been investigated. In the presence of
low density lipoproteins (LDL) normal fibroblasts converted LDL cholesterol to the oxysterol
27-hydroxycholesterol in quantities apparently sufficient to down-regulate 3-hydroxy-3-
methylglutaryl coenzyme A (HMG-CoA) reductase. The production of 27-hydroxycholesterol
started 3-8 h after the addition of LDL to the incubation medium, and during this time the …