[HTML][HTML] Electron microscopy of native cartilage oligomeric matrix protein purified from the Swarm rat chondrosarcoma reveals a five-armed structure.
M Mörgelin, D Heinegård, J Engel… - Journal of biological …, 1992 - Elsevier
M Mörgelin, D Heinegård, J Engel, M Paulsson
Journal of biological chemistry, 1992•ElsevierCartilage oligomeric matrix protein was isolated in the native state from the Swarm rat
chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The
purified protein was subjected to electron microscopy using rotary shadowing and negative
stain. The images allowed the construction of a structural model. The bouquet-like protein
consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand,
and a central assembly domain, where the five arms meet in a cylindrical structure.
chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The
purified protein was subjected to electron microscopy using rotary shadowing and negative
stain. The images allowed the construction of a structural model. The bouquet-like protein
consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand,
and a central assembly domain, where the five arms meet in a cylindrical structure.
Cartilage oligomeric matrix protein was isolated in the native state from the Swarm rat chondrosarcoma. A crucial step was its selective extraction with EDTA-containing buffer. The purified protein was subjected to electron microscopy using rotary shadowing and negative stain. The images allowed the construction of a structural model. The bouquet-like protein consists of five 28-nm-long arms containing a peripheral globular domain, a flexible strand, and a central assembly domain, where the five arms meet in a cylindrical structure.
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