[HTML][HTML] Clathrin-and AP-2-binding sites in HIP1 uncover a general assembly role for endocytic accessory proteins
Clathrin-mediated endocytosis is a major pathway for the internalization of macromolecules
into the cytoplasm of eukaryotic cells. The principle coat components, clathrin and the AP-2
adaptor complex, assemble a polyhedral lattice at plasma membrane bud sites with the aid
of several endocytic accessory proteins. Here, we show that huntingtin-interacting protein 1
(HIP1), a binding partner of huntingtin, copurifies with brain clathrin-coated vesicles and
associates directly with both AP-2 and clathrin. The discrete interaction sequences within …
into the cytoplasm of eukaryotic cells. The principle coat components, clathrin and the AP-2
adaptor complex, assemble a polyhedral lattice at plasma membrane bud sites with the aid
of several endocytic accessory proteins. Here, we show that huntingtin-interacting protein 1
(HIP1), a binding partner of huntingtin, copurifies with brain clathrin-coated vesicles and
associates directly with both AP-2 and clathrin. The discrete interaction sequences within …