Inhibition of PKB/Akt1 by C2-ceramide involves activation of ceramide-activated protein phosphatase in PC12 cells
M Salinas, R López-Valdaliso, D Martı́n… - Molecular and Cellular …, 2000 - Elsevier
Accumulation of ceramide has been reported in stress-and receptor-induced apoptosis in
the nervous system. However, its role in apoptosis signaling remains elusive. We describe
here the inhibition of the NGF-activated phosphoinositide 3-kinase (PI3K)-PKB/Akt1 survival
pathway by the cell permeable analog C2-ceramide. C2-ceramide did not inhibit ERK, PI3K,
or PDK1 activities and did not alter the translocation of PDK1 and Akt1 to the plasma
membrane, but blocked nuclear translocation of Akt1. Down-regulation of the Akt pathway …
the nervous system. However, its role in apoptosis signaling remains elusive. We describe
here the inhibition of the NGF-activated phosphoinositide 3-kinase (PI3K)-PKB/Akt1 survival
pathway by the cell permeable analog C2-ceramide. C2-ceramide did not inhibit ERK, PI3K,
or PDK1 activities and did not alter the translocation of PDK1 and Akt1 to the plasma
membrane, but blocked nuclear translocation of Akt1. Down-regulation of the Akt pathway …