[HTML][HTML] Binding of glycosulfopeptides to P-selectin requires stereospecific contributions of individual tyrosine sulfate and sugar residues

A Leppänen, SP White, J Helin, RP McEver… - Journal of Biological …, 2000 - ASBMB
P-selectin glycoprotein ligand-1 (PSGL-1) is a mucin on leukocytes that binds to selectins. P-
selectin binds to an N-terminal region of PSGL-1 that requires sulfation of at least one of
three clustered tyrosines (TyrSO 3) and an adjacent core-2-based O-glycan expressing
sialyl Lewis x (C2-O-sLe x). We synthesized glycosulfopeptides (GSPs) modeled after this
region of PSGL-1 to explore the roles of individual TyrSO 3 residues, the placement of C2-O-
sLe x relative to TyrSO 3, the relative contributions of fucose and sialic acid on C2-O-sLe x …