CFU-S in individual erythroid colonies derived in vitro from adult mouse marrow

RK Humphries, PB Jacky, FJ Dill, AC Eaves, CJ Eaves - Nature, 1979 - nature.com
RK Humphries, PB Jacky, FJ Dill, AC Eaves, CJ Eaves
Nature, 1979nature.com
IT is generally accepted that in the haematopoietic system there is a hierarchy of pluripotent
and committed progenitor cell types. Representative committed progenitors from all the
myeloid and lymphoid pathways can be detected by in vitro colony assays1. This
methodology has been extended to include multipotent haematopoietic progenitors2–5, and
it has since been shown that at least one such multipotent cell type, the progenitor of
macroscopic erythroid-megakaryocyte bursts, undergoes self-renewal during colony …
Abstract
IT is generally accepted that in the haematopoietic system there is a hierarchy of pluripotent and committed progenitor cell types. Representative committed progenitors from all the myeloid and lymphoid pathways can be detected by in vitro colony assays1. This methodology has been extended to include multipotent haematopoietic progenitors2–5, and it has since been shown that at least one such multipotent cell type, the progenitor of macroscopic erythroid-megakaryocyte bursts, undergoes self-renewal during colony formation in vitro5. We now report that cells capable of macroscopic spleen colony formation (CFU-S)6 are present in macroscopic bursts and that on average two to three and up to five self-renewal divisions of CFU-S can occur during the first 9 d of burst growth. These findings raise the possibility that clonal analysis techniques7 may be combined with cell culture manipulations to investigate how the choice between self-renewal and progressive differentiation is determined.
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