Induction of Colonies of Hemoglobin-Synthesizing Cells by Erythropoietin In Vitro

JR Stephenson, AA Axelrad… - Proceedings of the …, 1971 - National Acad Sciences
JR Stephenson, AA Axelrad, DL McLeod, MM Shreeve
Proceedings of the National Academy of Sciences, 1971National Acad Sciences
A culture method has been developed in which erythroid colonies are produced in vitro from
hemopoietic cells from the livers of 13-day fetuses of C3Hf/Bi mice. Heme synthesis by the
cultures was correlated with the presence of these colonies, and the hemoglobin produced
was shown to be electrophoretically normal. The individual colonies were identified as
erythroid since they were erythropoietin-dependent, positively stained by the histochemical
“Lepehne” procedure for hemoglobin, and labeled by 59Fe radioautography. Evidence is …
A culture method has been developed in which erythroid colonies are produced in vitro from hemopoietic cells from the livers of 13-day fetuses of C3Hf/Bi mice. Heme synthesis by the cultures was correlated with the presence of these colonies, and the hemoglobin produced was shown to be electrophoretically normal. The individual colonies were identified as erythroid since they were erythropoietin-dependent, positively stained by the histochemical “Lepehne” procedure for hemoglobin, and labeled by 59Fe radioautography. Evidence is presented that the development of these colonies is under separate control from that of granulocytic colonies found in the same cultures.
National Acad Sciences