Induction of megakaryocyte colonies with platelet formation in vitro

DL McLeod, MM Shreeve, AA Axelrad - Nature, 1976 - nature.com
DL McLeod, MM Shreeve, AA Axelrad
Nature, 1976nature.com
COLONY culture methods utilising semi-solid media which facilitate extensive cellular
differentiation in vitro have proved invaluable for the quantitative investigation of
granulocytic and erythrocytic progenitors and the effector substances to which they
respond1, 2. Colonies of megakaryocytes have been produced from suspensions of mouse
bone marrow or spleen cells in agar containing lymphocyte-conditioned medium3. But these
megakaryocytes did not reach the stage of maturation at which significant platelet production …
Abstract
COLONY culture methods utilising semi-solid media which facilitate extensive cellular differentiation in vitro have proved invaluable for the quantitative investigation of granulocytic and erythrocytic progenitors and the effector substances to which they respond1,2. Colonies of megakaryocytes have been produced from suspensions of mouse bone marrow or spleen cells in agar containing lymphocyte-conditioned medium3. But these megakaryocytes did not reach the stage of maturation at which significant platelet production could be demonstrated. We now report the development of megakaryocyte colonies with extensive platelet production in plasma cultures seeded with mouse bone marrow cells and containing either sheep or human erythropoietin in the medium. The proportional relation observed between the number of colonies produced and the number of bone marrow cells plated also provides the basis of a quantitative assay method for megakaryocyte progenitors in murine haemopoietic tissues.
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