A direct measurement of the radiation sensitivity of normal mouse bone marrow cells

JE Till, EA McCulloch - Radiation research, 1961 - JSTOR
JE Till, EA McCulloch
Radiation research, 1961JSTOR
Evidence is accumulating that the proliferative capacity of mammalian a uniformly high
radiation sensitivity regardless of the species and tissue of The evidence derives from
experiments on fresh explants and established in tissue culture (1-4), and on transplantable
tumors in vivo (5) where si techniques have been applied. Further, experiments using an
indirect tech measure the sensitivity of normal mouse bone marrow indicated that th have a
radiation sensitivity of similar magnitude (6). In the present repor method of assay for these …
Evidence is accumulating that the proliferative capacity of mammalian a uniformly high radiation sensitivity regardless of the species and tissue of The evidence derives from experiments on fresh explants and established in tissue culture (1-4), and on transplantable tumors in vivo (5) where si techniques have been applied. Further, experiments using an indirect tech measure the sensitivity of normal mouse bone marrow indicated that th have a radiation sensitivity of similar magnitude (6). In the present repor method of assay for these cells with a single-cell technique will be describ The method is based on the fact that the intravenous injection of an app number of marrow cells into isologous hosts previously exposed to supr total-body irradiation leads to the formation of colonies of proliferating cells spleens of these animals. These colonies appear as gross nodules in the sple may readily be counted. The relationship between the number of cells inje the number of colonies appearing in the spleen has been determined and study the sensitivity to radiation of the proliferative capacity in vivo of adult mouse bone marrow cells irradiated in vitro. The results show that normal mouse bone marrow cells have a similar radiation sensitivity to other mammalian cells tested by very different methods.
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