[HTML][HTML] Role of IKKγ/NEMO in assembly of the IκB kinase complex
IKKγ/NEMO is a protein that is critical for the assembly of the high molecular weight IκB
kinase (IKK) complex. To investigate the role of IKKγ/NEMO in the assembly of the IKK
complex, we conducted a series of experiments in which the chromatographic distribution of
extracts prepared from cells transiently expressing epitope-tagged IKKγ/NEMO and the IKKs
were examined. When expressed alone following transfection, IKKα and IKKβ were present
in low molecular weight complexes migrating between 200 and 400 kDa. However, when …
kinase (IKK) complex. To investigate the role of IKKγ/NEMO in the assembly of the IKK
complex, we conducted a series of experiments in which the chromatographic distribution of
extracts prepared from cells transiently expressing epitope-tagged IKKγ/NEMO and the IKKs
were examined. When expressed alone following transfection, IKKα and IKKβ were present
in low molecular weight complexes migrating between 200 and 400 kDa. However, when …