[HTML][HTML] Correlation between expression of 5-lipoxygenase-activating protein, 5-lipoxygenase, and cellular leukotriene synthesis.

GK Reid, S Kargman, PJ Vickers, JA Mancini… - Journal of Biological …, 1990 - Elsevier
GK Reid, S Kargman, PJ Vickers, JA Mancini, C Leveille, D Ethier, DK Miller, JW Gillard
Journal of Biological Chemistry, 1990Elsevier
Previous studies involving transfection of cDNAs for 5-lipoxygenase-activating protein
(FLAP) and 5-lipoxygenase into osteosarcoma cells have shown that both these proteins are
essential for leukotriene synthesis (Dixon, RAF, Diehl, RE, Opas, E., Rands, E., Vickers, PJ,
Evans, JF, Gillard, JW, and Miller, DK (1990) Nature 343, 282-284). In the present study we
show that FLAP is present in a variety of cells known to produce leukotrienes, but is absent
from a number of cells which do not synthesize leukotrienes. Furthermore, differentiation of …
Previous studies involving transfection of cDNAs for 5-lipoxygenase-activating protein (FLAP) and 5-lipoxygenase into osteosarcoma cells have shown that both these proteins are essential for leukotriene synthesis (Dixon, R. A. F., Diehl, R. E., Opas, E., Rands, E., Vickers, P. J., Evans, J. F., Gillard, J. W., and Miller, D. K. (1990) Nature 343, 282-284). In the present study we show that FLAP is present in a variety of cells known to produce leukotrienes, but is absent from a number of cells which do not synthesize leukotrienes. Furthermore, differentiation of the human promyelocytic HL-60 cell line towards granulocytic cells following exposure to dimethylsulfoxide is associated with the concurrent induction of both FLAP and 5-lipoxygenase and an increased capacity to synthesize leukotrienes. Cellular leukotriene synthesis in this system is functionally dependent on FLAP as shown by its inhibition by the leukotriene biosynthesis inhibitor MK-886, a compound which specifically binds to FLAP.
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