Transcellular conversion of endogenous arachidonic acid to lipoxins in mixed human platelet-granulocyte suspensions
C Edenius, J Haeggström, JÅ Lindgren - Biochemical and biophysical …, 1988 - Elsevier
C Edenius, J Haeggström, JÅ Lindgren
Biochemical and biophysical research communications, 1988•ElsevierIncubation of mixed human platelet/granulocyte suspensions with ionophore A23187 led to
a platelet dependent formation of several lipoxin isomers from endogenous substrate. The
major metabolite coeluted with authentic lipoxin A 4 (5 (S), 6 (R), 15 (S)-trihydroxy-7, 9, 13-
trans-11-cis-eicosatetraenoic acid) in several HPLC-systems and showed an identical UV-
spectrum. Furthermore, a similar profile of lipoxins was formed in pure platelet suspensions
incubated with exogenous leukotriene A 4 (5 (S)-5, 6-oxido-7, 9-trans-11, 14-cis …
a platelet dependent formation of several lipoxin isomers from endogenous substrate. The
major metabolite coeluted with authentic lipoxin A 4 (5 (S), 6 (R), 15 (S)-trihydroxy-7, 9, 13-
trans-11-cis-eicosatetraenoic acid) in several HPLC-systems and showed an identical UV-
spectrum. Furthermore, a similar profile of lipoxins was formed in pure platelet suspensions
incubated with exogenous leukotriene A 4 (5 (S)-5, 6-oxido-7, 9-trans-11, 14-cis …
Summary
Incubation of mixed human platelet/granulocyte suspensions with ionophore A23187 led to a platelet dependent formation of several lipoxin isomers from endogenous substrate. The major metabolite coeluted with authentic lipoxin A4 (5(S),6(R),15(S)-trihydroxy-7,9,13-trans-11-cis-eicosatetraenoic acid) in several HPLC-systems and showed an identical UV-spectrum. Furthermore, a similar profile of lipoxins was formed in pure platelet suspensions incubated with exogenous leukotriene A4 (5(S)-5,6-oxido-7,9-trans-11,14-cis-eicosatetraenoic acid). The conversion of exogenous leukotriene A4 to lipoxin A4 was markedly increased in the presence of ionophore A23187.
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