[HTML][HTML] Tyrosine Nitration by Simultaneous Generation of⋅ NO and O⨪ 2 under Physiological Conditions: HOW THE RADICALS DO THE JOB
S Goldstein, G Czapski, J Lind, G Merényi - Journal of Biological Chemistry, 2000 - ASBMB
Radiation chemical experiments demonstrate that the reaction of tyrosyl radical (TyrO⋅)
with⋅ NO 2 yields 45±3% 3-nitrotyrosine and that a major product of the reaction of TyrO⋅
with⋅ NO is 3, 3′-dityrosine. Radiolysis was used to generate⋅ NO and O⨪ 2 in the
presence of tyrosine and bicarbonate at pH 7.5±0.1. The nitration yield was found to be dose
rate-dependent, and the yield per radical produced by pulse radiolysis was identical to that
obtained with authentic peroxynitrite. The proposed mechanism that accounts for the data is …
with⋅ NO 2 yields 45±3% 3-nitrotyrosine and that a major product of the reaction of TyrO⋅
with⋅ NO is 3, 3′-dityrosine. Radiolysis was used to generate⋅ NO and O⨪ 2 in the
presence of tyrosine and bicarbonate at pH 7.5±0.1. The nitration yield was found to be dose
rate-dependent, and the yield per radical produced by pulse radiolysis was identical to that
obtained with authentic peroxynitrite. The proposed mechanism that accounts for the data is …