Comparative analysis of murine dendritic cells derived from spleen and bone marrow
RC Fields, JJ Osterholzer, JA Fuller… - Journal of …, 1998 - journals.lww.com
In order to improve upon preclinical tumor vaccine strategies that employ dendritic cells
(DC), we now have compared short-term cultures of spleen-and GMCSF/IL-4-stimulated
bone marrow (BM) to determine if differences exist in phenotype and function of murine DC
derived from primary and secondary hematolymphoid organs. Although cultures of BM
contained a lower percentage of DC compared to spleen, their capacity to stimulate a
primary allogeneic mixed leukocyte reaction (MLR) and to uptake fluorescent dextran was …
(DC), we now have compared short-term cultures of spleen-and GMCSF/IL-4-stimulated
bone marrow (BM) to determine if differences exist in phenotype and function of murine DC
derived from primary and secondary hematolymphoid organs. Although cultures of BM
contained a lower percentage of DC compared to spleen, their capacity to stimulate a
primary allogeneic mixed leukocyte reaction (MLR) and to uptake fluorescent dextran was …