A mouse hepatoma cell line which secretes several serum proteins including albumin and α-foetoprotein
C Szpirer, J Szpirer - Differentiation, 1975 - Elsevier
A permanent cell line (BW) was established from a transplantable mouse hepatoma,
BW7756, which produces α-foetoprotein (AFP). Three clones were isolated from the
uncloned culture: BW1, BW2 and B WTG3. The cells of the latter clone, which was isolated
after selection in the presence of thioguanine, are deficient in the enzyme hypoxanthine-
guanine-phosphoribosyl transferase. Both B W1 and BWTG3 cells have mean chromosome
number of 64 (60 telocentric and 4 metacentric chromosomes). All three clones secrete at …
BW7756, which produces α-foetoprotein (AFP). Three clones were isolated from the
uncloned culture: BW1, BW2 and B WTG3. The cells of the latter clone, which was isolated
after selection in the presence of thioguanine, are deficient in the enzyme hypoxanthine-
guanine-phosphoribosyl transferase. Both B W1 and BWTG3 cells have mean chromosome
number of 64 (60 telocentric and 4 metacentric chromosomes). All three clones secrete at …