Identification of a novel receptor/signal transduction pathway for IL‐15/T in mast cells.
Y Tagaya, JD Burton, Y Miyamoto… - The EMBO journal, 1996 - embopress.org
Y Tagaya, JD Burton, Y Miyamoto, TA Waldmann
The EMBO journal, 1996•embopress.orgInterleukin‐15/T (IL‐15) is a growth factor that utilizes IL‐2 receptor (IL‐2R) components in
addition to its private binding protein IL‐15R (alpha) in T‐cells. Here, we report that IL‐15
induces mast cell proliferation in the absence of IL‐2R alpha and beta. Using transfectants
of these cells with a cytoplasmic‐truncated mutant of gamma (c), we demonstrated that IL‐15
signaling in mast cells does not involve gamma (c). Cross‐linking of mast cells with [(125) I]
IL‐15 revealed a 60–65 kDa IL‐15 binding protein that is distinct from known components of …
addition to its private binding protein IL‐15R (alpha) in T‐cells. Here, we report that IL‐15
induces mast cell proliferation in the absence of IL‐2R alpha and beta. Using transfectants
of these cells with a cytoplasmic‐truncated mutant of gamma (c), we demonstrated that IL‐15
signaling in mast cells does not involve gamma (c). Cross‐linking of mast cells with [(125) I]
IL‐15 revealed a 60–65 kDa IL‐15 binding protein that is distinct from known components of …
Interleukin‐15/T(IL‐15) is a growth factor that utilizes IL‐2 receptor (IL‐2R) components in addition to its private binding protein IL‐15R(alpha) in T‐cells. Here, we report that IL‐15 induces mast cell proliferation in the absence of IL‐2R alpha and beta. Using transfectants of these cells with a cytoplasmic‐truncated mutant of gamma(c), we demonstrated that IL‐15 signaling in mast cells does not involve gamma(c). Cross‐linking of mast cells with [(125)I]IL‐15 revealed a 60–65 kDa IL‐15 binding protein that is distinct from known components of T‐cell IL‐15 receptors. Mast cell IL‐15 receptors recruit JAK‐2 and STAT‐5, instead of JAK1/3 and STAT3/5 that are activated in T‐cells. Thus IL‐15 is a mast cell growth factor that utilizes a novel receptor and distinct signaling pathway.
embopress.org