Cloning of murine and rat vascular cell adhesion molecule-1
C Hession, P Moy, R Tizard, P Chisholm… - Biochemical and …, 1992 - Elsevier
C Hession, P Moy, R Tizard, P Chisholm, C Williams, M Wysk, L Burkly, K Miyake, P Kincade…
Biochemical and biophysical research communications, 1992•ElsevierVascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig)
superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the
cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both
sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and
76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show
VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two …
superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the
cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both
sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and
76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show
VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two …
Vascular cell adhesion molecule-1 (VCAM1) is a member of the immunoglobulin (Ig) superfamily which interacts with the integrin very late antigen 4 (VLA4). We have cloned the cDNAs for both murine and rat VCAM1 from endotoxin-treated lung libraries. Both sequences encode proteins with seven extracellular Ig-like domains, which show 75.9% and 76.9% identity, respectively, with human VCAM1. Both murine and human cell lines show VLA4-dependent binding to COS cells transiently expressing murine and rat VCAM1. Two mAbs, MK 1 and MK 2, which recognize an antigen on murine bone marrow stromal cell lines, bind to murine VCAM1 expressed in COS cells and block VCAM1-dependent adhesion, confirming that these mAbs recognize murine VCAM1.
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