A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells
P Hunt, D Robertson, D Weiss, D Rennick, F Lee… - Cell, 1987 - cell.com
P Hunt, D Robertson, D Weiss, D Rennick, F Lee, ON Witte
Cell, 1987•cell.comA clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting
the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-
promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor,
both of which accumulate in ALC-culture supernatant. To analyze the lymphold growth factor
produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-
growthconditioned medium. Using a combination of biological and biochemical analyses …
the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-
promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor,
both of which accumulate in ALC-culture supernatant. To analyze the lymphold growth factor
produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-
growthconditioned medium. Using a combination of biological and biochemical analyses …
Summary
A clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor, both of which accumulate in ALC-culture supernatant. To analyze the lymphold growth factor produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-growthconditioned medium. Using a combination of biological and biochemical analyses, we have established that the pre-B cell growth factor produced by ALC cells is distinct from IL-l, IL-2, IL-3, and IL-4 (BSF-l), suggesting that the early stages of B-cell development are regulated by a unique stroma-derived growth factor.
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