A single bone marrow-derived stromal cell type supports the in vitro growth of early lymphoid and myeloid cells

P Hunt, D Robertson, D Weiss, D Rennick, F Lee… - Cell, 1987 - cell.com
P Hunt, D Robertson, D Weiss, D Rennick, F Lee, ON Witte
Cell, 1987cell.com
A clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting
the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-
promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor,
both of which accumulate in ALC-culture supernatant. To analyze the lymphold growth factor
produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-
growthconditioned medium. Using a combination of biological and biochemical analyses …
Summary
A clonal cell line (ALC) derived from murine bone marrow stroma is capable of supporting the continuous, in vitro growth of early lymphoid and myeloid cell populations. The growth-promoting effects of ALC are in part mediated through M-CSF and a pre-B cell growth factor, both of which accumulate in ALC-culture supernatant. To analyze the lymphold growth factor produced by ALC cells, we derived a pre-B cell indicator line that is dependent on ALC-growthconditioned medium. Using a combination of biological and biochemical analyses, we have established that the pre-B cell growth factor produced by ALC cells is distinct from IL-l, IL-2, IL-3, and IL-4 (BSF-l), suggesting that the early stages of B-cell development are regulated by a unique stroma-derived growth factor.
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