[HTML][HTML] CD95 (Fas/APO-1) signals ceramide generation independent of the effector stage of apoptosis
C Grullich, MC Sullards, Z Fuks, AH Merrill… - Journal of Biological …, 2000 - ASBMB
Although numerous studies document caspase-independent ceramide generation
preceding apoptosis upon environmental stress, the molecular ordering of ceramide
generation during cytokine-induced apoptosis remains uncertain. Here, we show that CD95-
induced ceramide elevation occurs during the initiation phase of apoptosis. We titrated down
the amount of FADD transfected into HeLa and 293T cells until it was insufficient for
apoptosis, although cycloheximide (CHX) still triggered the effector phase. Even in the …
preceding apoptosis upon environmental stress, the molecular ordering of ceramide
generation during cytokine-induced apoptosis remains uncertain. Here, we show that CD95-
induced ceramide elevation occurs during the initiation phase of apoptosis. We titrated down
the amount of FADD transfected into HeLa and 293T cells until it was insufficient for
apoptosis, although cycloheximide (CHX) still triggered the effector phase. Even in the …