[HTML][HTML] Involvement of sphingosine in mitochondria-dependent Fas-induced apoptosis of type II Jurkat T cells
O Cuvillier, L Edsall, S Spiegel - Journal of Biological Chemistry, 2000 - ASBMB
Exposure to anti-Fas antibody in Jurkat cells (type II cells), which are characterized by a
weak caspase-8 activation at the death-inducing signaling complex (DISC), induced a
biphasic increase in ceramide levels. The early generation of ceramide preceded transient
activation of acidic ceramidase and subsequent production of sphingosine, followed by
cytochrome c release, activation of caspases-2,-3,-6,-7,-8, and-9, Bid cleavage, and a later
sustained ceramide accumulation. The caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp …
weak caspase-8 activation at the death-inducing signaling complex (DISC), induced a
biphasic increase in ceramide levels. The early generation of ceramide preceded transient
activation of acidic ceramidase and subsequent production of sphingosine, followed by
cytochrome c release, activation of caspases-2,-3,-6,-7,-8, and-9, Bid cleavage, and a later
sustained ceramide accumulation. The caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp …