Genetic and biochemical analysis of Shigella dysenteriae 1 O antigen polysaccharide biosynthesis in Escherichia coli K-12: structure and functions of the rfb gene …

S Sturm, B Jann, K Jann, P Fortnagel, KN Timmis - Microbial pathogenesis, 1986 - Elsevier
The genetic organization and functions of the Shigella dysenteriae 1 rfb gene cluster, which
specifies the somatic O antigen in this organism, have been studied in Escherichia coli K-12
by insertion and deletion mutagenesis of pSS9, a pBR322 hybrid containing the Shigella rfb
genes. On the basis of the sensitivity/resistance to rough-specific bacteriophage T3 of E. coli
K-12 derivatives containing mutant pSS9 plasmids, of the banding patterns and
immunoreactivity of LPS isolated from such derivatives and electrophoresed on SDS …