The large Bacillus plasmid pTB19 contains two integrated rolling-circle plasmids carrying mobilization functions
L Oskam, DJ Hillenga, G Venema, S Bron - Plasmid, 1991 - Elsevier
Plasmid pTB19 is a 27-kb plasmid originating from a thermophilic Bacillus species. It was
shown previously that pTB19 contains an integrated copy of the rolling-circle type plasmid
pTB913. Here we describe the analysis of a 4324-bp region of pTB19 conferring resistance
to tetracycline. The nucleotide sequence of this region revealed all the characteristics of a
second plasmid replicating via the rolling-circle mechanism. This sequence contained (i) the
tetracycline resistance marker of pTB19, which is highly similar to other tetL-genes of gram …
shown previously that pTB19 contains an integrated copy of the rolling-circle type plasmid
pTB913. Here we describe the analysis of a 4324-bp region of pTB19 conferring resistance
to tetracycline. The nucleotide sequence of this region revealed all the characteristics of a
second plasmid replicating via the rolling-circle mechanism. This sequence contained (i) the
tetracycline resistance marker of pTB19, which is highly similar to other tetL-genes of gram …