Cellular origin and distribution of transforming growth factor-β1 in the normal rat myocardium
M Eghbali - Cell and tissue research, 1989 - Springer
M Eghbali
Cell and tissue research, 1989•SpringerTransforming growth factor-β (TGF-β) is a biologically active polypeptide present in normal
tissues as well as transformed cells. Two structurally related forms of this peptide are TGF-β
1 and TGF-β 2. Using freshly isolated cardiomyocytes and non-myocyte heart cells, and a
[32 P]-labelled cDNA probe to human TGF-β 1, we demonstrated that mRNA for TGF-β 1
could be detected only in the nonmyocyte fraction of heart cells. In the present study, the
distribution of TGF-β 1 in the heart was determined by immunofluorescence staining by use …
tissues as well as transformed cells. Two structurally related forms of this peptide are TGF-β
1 and TGF-β 2. Using freshly isolated cardiomyocytes and non-myocyte heart cells, and a
[32 P]-labelled cDNA probe to human TGF-β 1, we demonstrated that mRNA for TGF-β 1
could be detected only in the nonmyocyte fraction of heart cells. In the present study, the
distribution of TGF-β 1 in the heart was determined by immunofluorescence staining by use …
Summary
Transforming growth factor-β (TGF-β) is a biologically active polypeptide present in normal tissues as well as transformed cells. Two structurally related forms of this peptide are TGF-β 1 and TGF-β 2. Using freshly isolated cardiomyocytes and non-myocyte heart cells, and a [32P]-labelled cDNA probe to human TGF-β 1, we demonstrated that mRNA for TGF-β 1 could be detected only in the nonmyocyte fraction of heart cells. In the present study, the distribution of TGF-β 1 in the heart was determined by immunofluorescence staining by use of a polyclonal antibody to porcine TGF-β 1 in cryostat sections of rat heart. Immunofluorescence staining was intense around the blood vessels and radially diffuse in the surrounding myocardium.
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