Structure identification of the complex-type, asparagine-linked sugar chains of β-D-galactosyl-transferase purified from human milk
T Endo, J Amano, EG Berger, A Kobata - Carbohydrate research, 1986 - Elsevier
The asparagine-linked sugar chains of human milk galactosyltansferase were quantitatively
released as oligosaccharides from the polypeptide backbone by hydrazinolysis. They were
converted into radioactive oligosaccharides by sodium borotritiate reduction after N-
acetylation, and fractionated by paper electrophoresis and by Bio-Gel P-4 column
chromatography after sialidase treatment. Structural studies of each oligosaccharides by
sequential exoglycosidase digestion and methylation analysis indicated that the …
released as oligosaccharides from the polypeptide backbone by hydrazinolysis. They were
converted into radioactive oligosaccharides by sodium borotritiate reduction after N-
acetylation, and fractionated by paper electrophoresis and by Bio-Gel P-4 column
chromatography after sialidase treatment. Structural studies of each oligosaccharides by
sequential exoglycosidase digestion and methylation analysis indicated that the …