[HTML][HTML] Chinese hamster ovary (CHO) cells may express six β4-galactosyltransferases (β4GalTs): consequences of the loss of functional β4GalT-1, β4GalT-6, or both …
JH Lee, S Sundaram, NL Shaper, TS Raju… - Journal of Biological …, 2001 - ASBMB
Six β4-galactosyltransferase (β4GalT) genes have been cloned from mammalian sources.
We show that all six genes are expressed in the Gat− 2 line of Chinese hamster ovary cells
(Gat− 2 CHO). Two independent mutants termed Pro− 5Lec20 and Gat− 2Lec20, previously
selected for lectin resistance, were found to have a galactosylation defect. Radiolabeled
biantennary N-glycans synthesized by Pro− 5Lec20 were proportionately less ricin-bound
than similar species from parental CHO cells, and Lec20 cell extracts had a markedly …
We show that all six genes are expressed in the Gat− 2 line of Chinese hamster ovary cells
(Gat− 2 CHO). Two independent mutants termed Pro− 5Lec20 and Gat− 2Lec20, previously
selected for lectin resistance, were found to have a galactosylation defect. Radiolabeled
biantennary N-glycans synthesized by Pro− 5Lec20 were proportionately less ricin-bound
than similar species from parental CHO cells, and Lec20 cell extracts had a markedly …