Prostasin is a novel human serine proteinase from seminal fluid. Purification, tissue distribution, and localization in prostate gland.
JX Yu, L Chao, J Chao - Journal of Biological Chemistry, 1994 - ASBMB
A novel serine proteinase, designated as prostasin, has been purified from human seminal
fluid to apparent homogeneity by DEAE-Sepharose CL-6B and aprotinin-affinity
chromatography. The purified protein migrates as two close bands with an apparent
molecular mass of 40 kDa on SDS-polyacrylamide gel electrophoresis under reducing
conditions. It can be labeled with [14C] diisopropyl fluorophosphate and has a pI ranging
from 4.5 to 4.8. Sequence analysis reveals that the two protein bands have an identical NH2 …
fluid to apparent homogeneity by DEAE-Sepharose CL-6B and aprotinin-affinity
chromatography. The purified protein migrates as two close bands with an apparent
molecular mass of 40 kDa on SDS-polyacrylamide gel electrophoresis under reducing
conditions. It can be labeled with [14C] diisopropyl fluorophosphate and has a pI ranging
from 4.5 to 4.8. Sequence analysis reveals that the two protein bands have an identical NH2 …