Activation of recombinant murine cytotoxic cell proteinase-1 requires deletion of an amino-terminal dipeptide.
A Caputo, RS Garner, U Winkler, D Hudig… - Journal of Biological …, 1993 - ASBMB
Murine cytotoxic cell proteinase-1 (granzyme B) is a member of a family of novel serine
proteinases that have been implicated to participate in destruction of target cells by cytotoxic
T lymphocytes. Comparison of the sequence of the cDNA with the sequence of the protein
isolated from cytoplasmic granules of cytotoxic T lymphocytes suggested that this protein
may be synthesized as a preproenzyme containing an amino-terminal activation dipeptide.
Here we show that this activation dipeptide regulates the activity of the enzyme in hydrolysis …
proteinases that have been implicated to participate in destruction of target cells by cytotoxic
T lymphocytes. Comparison of the sequence of the cDNA with the sequence of the protein
isolated from cytoplasmic granules of cytotoxic T lymphocytes suggested that this protein
may be synthesized as a preproenzyme containing an amino-terminal activation dipeptide.
Here we show that this activation dipeptide regulates the activity of the enzyme in hydrolysis …