[HTML][HTML] Localization of LAT in glycolipid-enriched microdomains is required for T cell activation
LAT, a transmembrane adapter protein found in glycolipid-enriched microdomains (GEMs),
is essential for T cell activation. In this study, we have utilized a LAT-deficient mutant of the
Jurkat T cell line, J. CaM2, to explore various requirements for LAT function. First, we
demonstrate that LAT must be present in GEMs for coupling T cell receptor (TCR)
engagement to activation of the Ras signaling pathway, increases in intracellular Ca 2+, and
induction of the transcription factor nuclear factor of activated T cells (NF-AT). Second, we …
is essential for T cell activation. In this study, we have utilized a LAT-deficient mutant of the
Jurkat T cell line, J. CaM2, to explore various requirements for LAT function. First, we
demonstrate that LAT must be present in GEMs for coupling T cell receptor (TCR)
engagement to activation of the Ras signaling pathway, increases in intracellular Ca 2+, and
induction of the transcription factor nuclear factor of activated T cells (NF-AT). Second, we …