Structural elucidation of the disulfated oligosaccharide from bovine lutropin.
ED Green, H Van Halbeek, I Boime… - Journal of Biological …, 1985 - ASBMB
The Asn-linked oligosaccharides of the pituitary hormone lutropin (LH) contain both sulfate
and GalNAc. Bovine pituitary explants incorporate [3H] glucosamine,[3H] mannose,[3H]
fucose, and [35S] sulfate into the Asn-linked oligosaccharides of LH. Endoglycosidase F or
N-glycanase releases the [3H] glucosamine-and [3H] mannose-labeled oligosaccharides
from the protein, which resolve on anion-exchange high pressure liquid chromatography as
neutral (S-0), mono-(S-1), and disulfated (S-2) species. Based on sequential enzyme …
and GalNAc. Bovine pituitary explants incorporate [3H] glucosamine,[3H] mannose,[3H]
fucose, and [35S] sulfate into the Asn-linked oligosaccharides of LH. Endoglycosidase F or
N-glycanase releases the [3H] glucosamine-and [3H] mannose-labeled oligosaccharides
from the protein, which resolve on anion-exchange high pressure liquid chromatography as
neutral (S-0), mono-(S-1), and disulfated (S-2) species. Based on sequential enzyme …