[HTML][HTML] Isolation of a cDNA encoding a novel member of the transglutaminase gene family from human keratinocytes: detection and identification of transglutaminase …
D Aeschlimann, MK Koeller, BL Allen-Hoffmann… - Journal of Biological …, 1998 - ASBMB
We developed a method using a single set of degenerate oligonucleotide primers for
amplification of the conserved active site of transglutaminases by reverse transcription-
polymerase chain reaction (RT-PCR) and identification of the PCR products by cleavage
with diagnostic restriction enzymes. We demonstrate amplification of tissue
transglutaminase (TG C), keratinocyte transglutaminase (TG K), prostate transglutaminase
(TG P), the a-subunit of factor XIII, and band 4.2 protein from different human cells or tissues …
amplification of the conserved active site of transglutaminases by reverse transcription-
polymerase chain reaction (RT-PCR) and identification of the PCR products by cleavage
with diagnostic restriction enzymes. We demonstrate amplification of tissue
transglutaminase (TG C), keratinocyte transglutaminase (TG K), prostate transglutaminase
(TG P), the a-subunit of factor XIII, and band 4.2 protein from different human cells or tissues …