Heparin cofactor II. Purification and properties of a heparin-dependent inhibitor of thrombin in human plasma.
DM Tollefsen, DW Majerus, MK Blank - Journal of Biological Chemistry, 1982 - ASBMB
We have isolated a previously unrecognized heparin-dependent inhibitor of thrombin from
human plasma. The inhibitor, designated heparin cofactor II (HCII), was purified to
homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-
150. HCII is a glycoprotein consisting of a single polypeptide chain with a Mr= 65,600 as
determined by sedimentation equilibrium analysis. Other physical properties include s20, w=
4.31 S; Stokes radius= 34 A; E280 1%= 11.7; and pI= 4.95 to 5.15. The purified inhibitor is …
human plasma. The inhibitor, designated heparin cofactor II (HCII), was purified to
homogeneity with sulfated-dextran, DEAE-Sepharose, heparin-Sepharose and Sephadex G-
150. HCII is a glycoprotein consisting of a single polypeptide chain with a Mr= 65,600 as
determined by sedimentation equilibrium analysis. Other physical properties include s20, w=
4.31 S; Stokes radius= 34 A; E280 1%= 11.7; and pI= 4.95 to 5.15. The purified inhibitor is …