Receptor binding of pancreatic spasmolytic polypeptide in intestinal mucosal cells and membranes
EK Frandsen - Regulatory peptides, 1988 - Elsevier
Rat intestinal mucosal cells contain receptors for pancreatic spasmolytic polypeptide (PSP).
The binding of 125I-PSP was rapid, saturable, reversible and specific. PSP competed with
125I-PSP for binding to the receptors and 10− 7 M of PSP half-maximally inhibited 125I-PSP
binding. The normalized PSP dose-response graphs in intact cells and crude membranes
were superimposable. Scatchard plots of PSP binding to membranes were curvilinear,
indicating multiple classes of binding sites, negative cooperative interaction between sites or …
The binding of 125I-PSP was rapid, saturable, reversible and specific. PSP competed with
125I-PSP for binding to the receptors and 10− 7 M of PSP half-maximally inhibited 125I-PSP
binding. The normalized PSP dose-response graphs in intact cells and crude membranes
were superimposable. Scatchard plots of PSP binding to membranes were curvilinear,
indicating multiple classes of binding sites, negative cooperative interaction between sites or …