Mechanisms of NSAID-induced gastrointestinal injury defined using mutant mice
PL Beck, R Xavier, N Lu, NN Nanda, M Dinauer… - Gastroenterology, 2000 - Elsevier
PL Beck, R Xavier, N Lu, NN Nanda, M Dinauer, DK Podolsky, B Seed
Gastroenterology, 2000•ElsevierBackground & Aims: Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used
agents that have a high incidence of gastrointestinal side effects resulting in significant
morbidity and mortality. Leukocytes have been implicated in NSAID-induced injury, but the
mechanisms are unclear. We established a murine model of NSAID-induced gastrointestinal
damage to assess the roles of candidate gene products in the pathogenesis of this injury.
Methods: Indomethacin-induced gastrointestinal injury was assessed in wild-type and …
agents that have a high incidence of gastrointestinal side effects resulting in significant
morbidity and mortality. Leukocytes have been implicated in NSAID-induced injury, but the
mechanisms are unclear. We established a murine model of NSAID-induced gastrointestinal
damage to assess the roles of candidate gene products in the pathogenesis of this injury.
Methods: Indomethacin-induced gastrointestinal injury was assessed in wild-type and …
Background & Aims
Nonsteroidal anti-inflammatory drugs (NSAIDs) are commonly used agents that have a high incidence of gastrointestinal side effects resulting in significant morbidity and mortality. Leukocytes have been implicated in NSAID-induced injury, but the mechanisms are unclear. We established a murine model of NSAID-induced gastrointestinal damage to assess the roles of candidate gene products in the pathogenesis of this injury.
Methods
Indomethacin-induced gastrointestinal injury was assessed in wild-type and several mutant murine lines. Leukocyte involvement was assessed by neutrophil depletion, impairment of recruitment (resulting from targeted disruption of fucosyltransferase VII [FTVII]), and the absence of mature T and B cells with the use of Rag 2−/− mice. Activation and oxygen free radicals were assessed using gp91phox−/− mice that exhibit normal leukocyte recruitment but are deficient in myeloid cell activation and oxygen free radical generation.
Results
Impairment of leukocyte recruitment (FTVII/−) and neutrophil depletion resulted in more than a 50% reduction in NSAID-induced injury. However, mice deficient in mature T and B cells had NSAID-induced damage comparable to control mice. Leukocyte activation was required for NSAID-induced damage because the gp91phox−/− mice were less susceptible to NSAID injury than wild-type mice.
Conclusions
In this murine model system, FTVII-dependent leukocyte recruitment, leukocyte activation via gp91phox, and neutrophils are required for NSAID-induced gastrointestinal injury, whereas T and B cells are not essential.
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