[PDF][PDF] Control of human sebocyte proliferation in vitro by testosterone and 5-alpha-dihydrotestosterone is dependent on the localization of the sebaceous glands
H Akamatsu, CC Zouboulis, CE Orfanos - Journal of investigative …, 1992 - core.ac.uk
H Akamatsu, CC Zouboulis, CE Orfanos
Journal of investigative dermatology, 1992•core.ac.ukMATERIALS AND METHODS Human Sebocyte Cultures Skin specimens of the face and the
upper as well as the lower legs of five young patients (three male, ages 20, 22, and 23; two
female, ages 22 and 23) undergoing operation were used in this study. Primary sebocyte
cultures were obtained as previously described [4]. Briefly, full-thickness skin was incubated
for 20 h in 2.4 Ujml dispase (Boehringer, Mannheim, Germany) at 4 C to separate epidermis
from dermis. Epidermis was then maintained for 15 min in 0.02% desoxyribonuclease …
upper as well as the lower legs of five young patients (three male, ages 20, 22, and 23; two
female, ages 22 and 23) undergoing operation were used in this study. Primary sebocyte
cultures were obtained as previously described [4]. Briefly, full-thickness skin was incubated
for 20 h in 2.4 Ujml dispase (Boehringer, Mannheim, Germany) at 4 C to separate epidermis
from dermis. Epidermis was then maintained for 15 min in 0.02% desoxyribonuclease …
MATERIALS AND METHODS
Human Sebocyte Cultures Skin specimens of the face and the upper as well as the lower legs of five young patients (three male, ages 20, 22, and 23; two female, ages 22 and 23) undergoing operation were used in this study. Primary sebocyte cultures were obtained as previously described [4]. Briefly, full-thickness skin was incubated for 20 h in 2.4 Ujml dispase (Boehringer, Mannheim, Germany) at 4 C to separate epidermis from dermis. Epidermis was then maintained for 15 min in 0.02% desoxyribonuclease (Sigma, Deisenhofen, Germany) at 37 C. Intact sebaceous glands were isolated by using microsurgical instruments under microscopical ob-
core.ac.uk