Transmembrane phosphoprotein Cbp regulates the activities of Src-family tyrosine kinases

M Kawabuchi, Y Satomi, T Takao, Y Shimonishi… - Nature, 2000 - nature.com
M Kawabuchi, Y Satomi, T Takao, Y Shimonishi, S Nada, K Nagai, A Tarakhovsky, M Okada
Nature, 2000nature.com
The Src family of protein tyrosine kinases (Src-PTKs) is important in the regulation of growth
and differentiation of eukaryotic cells. The activity of Src-PTKs in cells of different types is
negatively controlled by Csk, which specifically phosphorylates a conserved regulatory
tyrosine residue at the carboxy-terminal tail of the Src-PTKs,,. Csk is mainly cytoplasmic and
Src-PTKs are predominantly membrane-associated. This raises a question about the
mechanism of interaction between these enzymes. Here we present Cbp—a …
Abstract
The Src family of protein tyrosine kinases (Src-PTKs) is important in the regulation of growth and differentiation of eukaryotic cells. The activity of Src-PTKs in cells of different types is negatively controlled by Csk, which specifically phosphorylates a conserved regulatory tyrosine residue at the carboxy-terminal tail of the Src-PTKs,,. Csk is mainly cytoplasmic and Src-PTKs are predominantly membrane-associated. This raises a question about the mechanism of interaction between these enzymes. Here we present Cbp—a transmembrane phosphoprotein that is ubiquitously expressed and binds specifically to the SH2 domain of Csk. Cbp is involved in the membrane localization of Csk and in the Csk-mediated inhibition of c-Src. In the plasma membrane Cbp is exclusively localized in the GM1 ganglioside-enriched detergent-insoluble membrane domain, which is important in receptor-mediated signalling,,,,. These findings reveal Cbp as a new component of the regulatory mechanism controlling the activity of membrane-associated Src-PTKs.
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