Ultrastructural localization of H+ ATPase in rabbit cortical collecting duct.

JW Verlander, KM Madsen, DK Stone… - Journal of the American …, 1994 - journals.lww.com
In contrast to results obtained in the rat kidney, studies of H+ ATPase localization in the
rabbit kidney have failed to demonstrate basolateral plasma membrane H+ ATPase
immunoreactivity in intercalated cells in the cortical collecting duct (CCD). Previous studies
have relied on light microscopic immunofluorescence techniques, which have limited
resolution. Therefore, the immunogold procedure was used to localize H+ ATPase in rabbit
collecting ducts at the ultrastructural level. Rabbit kidneys were preserved in vivo with …