Fluorescent indicators for cytosolic calcium based on rhodamine and fluorescein chromophores
A Minta, JPY Kao, RY Tsien - Journal of Biological Chemistry, 1989 - ASBMB
A new group of fluorescent indicators with visible excitation and emission wavelengths has
been synthesized for measurements of cytosolic free Ca 2+. The five compounds," rhod-1,""
rhod-2,"" fluo-1,"" fluo-2," and" fluo-3"(Figs. 2 and 3), combine the 8-coordinate
tetracarboxylate chelating site of 1, 2-bis (2-amino-phenoxyethane-N, N, N′, N′-
tetraacetic acid with a xanthene chromophore to give a rhodamine-like or fluorescein-like
fluorophore. Binding of Ca 2+ increases the fluorescence by up to 40-fold. The Ca 2+ …
been synthesized for measurements of cytosolic free Ca 2+. The five compounds," rhod-1,""
rhod-2,"" fluo-1,"" fluo-2," and" fluo-3"(Figs. 2 and 3), combine the 8-coordinate
tetracarboxylate chelating site of 1, 2-bis (2-amino-phenoxyethane-N, N, N′, N′-
tetraacetic acid with a xanthene chromophore to give a rhodamine-like or fluorescein-like
fluorophore. Binding of Ca 2+ increases the fluorescence by up to 40-fold. The Ca 2+ …