Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions
MG Redinbaugh, RB Turley - Analytical biochemistry, 1986 - Elsevier
Analytical biochemistry, 1986•Elsevier
The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a
plate reader to reduce the time needed for analyses. The total volume of the assay was
reduced to 0.21 ml with various ratios of sample to reagent volume being used. The assay is
sensitive to a limit of less than 1 μg of bovine serum albumin. The assay is insensitive to the
presence of the detergents sodium dodecyl sulfate, Triton X-100, and deoxycholate. In
addition, the method has been adapted for determining the protein concentrations of …
plate reader to reduce the time needed for analyses. The total volume of the assay was
reduced to 0.21 ml with various ratios of sample to reagent volume being used. The assay is
sensitive to a limit of less than 1 μg of bovine serum albumin. The assay is insensitive to the
presence of the detergents sodium dodecyl sulfate, Triton X-100, and deoxycholate. In
addition, the method has been adapted for determining the protein concentrations of …
The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a plate reader to reduce the time needed for analyses. The total volume of the assay was reduced to 0.21 ml with various ratios of sample to reagent volume being used. The assay is sensitive to a limit of less than 1 μg of bovine serum albumin. The assay is insensitive to the presence of the detergents sodium dodecyl sulfate, Triton X-100, and deoxycholate. In addition, the method has been adapted for determining the protein concentrations of sucrose density gradient fractions, which eliminates the need for removing the sucrose prior to assay.
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