Regulation of transepithelial ion transport and intracellular calcium by extracellular ATP in human normal and cystic fibrosis airway epithelium
SJ Mason, AM Paradiso… - British journal of …, 1991 - Wiley Online Library
SJ Mason, AM Paradiso, RC Boucher
British journal of pharmacology, 1991•Wiley Online Library1 The role of extracellular nucleotides in regulation of ion transport activities (short circuit
current, Isc) of human respiratory epithelia was studied. 2 Application of nucleotides to the
apical or basolateral membrane of human nasal epithelium induced a concentration‐
dependent increase in Isc. 3 The rank order of potency of purine‐or pyrimidine‐induced
changes in Isc of normal human nasal epithelium when applied to the apical membrane
(UTP≥ ATP> ATPγS> 2MeSATP> ADPβS≫ βγMeATP≥ βγMeATP) or basolateral …
current, Isc) of human respiratory epithelia was studied. 2 Application of nucleotides to the
apical or basolateral membrane of human nasal epithelium induced a concentration‐
dependent increase in Isc. 3 The rank order of potency of purine‐or pyrimidine‐induced
changes in Isc of normal human nasal epithelium when applied to the apical membrane
(UTP≥ ATP> ATPγS> 2MeSATP> ADPβS≫ βγMeATP≥ βγMeATP) or basolateral …
- 1The role of extracellular nucleotides in regulation of ion transport activities (short circuit current, Isc) of human respiratory epithelia was studied.
- 2Application of nucleotides to the apical or basolateral membrane of human nasal epithelium induced a concentration‐dependent increase in Isc.
- 3The rank order of potency of purine‐ or pyrimidine‐induced changes in Isc of normal human nasal epithelium when applied to the apical membrane (UTP ≥ ATP > ATPγS > 2MeSATP > ADPβS ≫ βγMeATP ≥ βγMeATP) or basolateral membrane (2MeSATP > UTP > ATP > ATPγS > αβMeATP > βγMeATP) is consistent with involvement of a P2 purinoceptor. A similar rank order of potencies was observed for nucleotide effects on intracellular calcium measured by Fura‐2 fluorescence using microspectrofluorimetry.
- 4Similar nucleotide potency in the regulation of ion transport and intracellular calcium in cystic fibrosis (CF) airway epithelium (UTP ≥ ATP) was observed, suggesting purinoceptors might be used to stimulate ion transport processes that would promote hydration of airway secretions and facilitate their clearance from CF lungs.
- 5These data provide evidence for the regulation of ion transport by P2 purinoceptors in normal and cystic fibrosis human airway epithelium.
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