[HTML][HTML] Calcium can disrupt the SNARE protein complex on sea urchin egg secretory vesicles without irreversibly blocking fusion
M Tahara, JR Coorssen, K Timmers, PS Blank… - Journal of Biological …, 1998 - ASBMB
The homotypic fusion of sea urchin egg cortical vesicles (CV) is a system in which to
correlate the biochemistry and physiology of membrane fusion. Homologues of vesicle-
associated membrane protein (VAMP), syntaxin, and SNAP-25 were identified in CV
membranes. A VAMP and syntaxin immunoreactive band at a higher apparent molecular
mass (≈ 70 kDa) was detected; extraction and analysis confirmed that the band contained
VAMP, SNAP-25, and syntaxin. This complex was also identified by immunoprecipitation …
correlate the biochemistry and physiology of membrane fusion. Homologues of vesicle-
associated membrane protein (VAMP), syntaxin, and SNAP-25 were identified in CV
membranes. A VAMP and syntaxin immunoreactive band at a higher apparent molecular
mass (≈ 70 kDa) was detected; extraction and analysis confirmed that the band contained
VAMP, SNAP-25, and syntaxin. This complex was also identified by immunoprecipitation …