[HTML][HTML] Phosphorylation of the Respiratory Burst Oxidase Subunit p47phox as Determined by Two-dimensional Phosphopeptide Mapping: PHOSPHORYLATION BY …
J El Benna, PF La Rosa, JL Johnson… - Journal of Biological …, 1996 - ASBMB
The respiratory burst oxidase is responsible for superoxide (O 2) production by phagocytes
and B lymphocytes. This multicomponent enzyme is dormant in resting cells but is activated
on exposure of the cells to an appropriate stimulus. Upon activation, several serine residues
on the cytosolic oxidase subunit p47 phox become phosphorylated. Using two-dimensional
tryptic phosphopeptide mapping, we studied the phosphorylation of p47 phox in 32 P i-
loaded Epstein-Barr virus-transformed B lymphoblasts expressing wild type p47 phox or any …
and B lymphocytes. This multicomponent enzyme is dormant in resting cells but is activated
on exposure of the cells to an appropriate stimulus. Upon activation, several serine residues
on the cytosolic oxidase subunit p47 phox become phosphorylated. Using two-dimensional
tryptic phosphopeptide mapping, we studied the phosphorylation of p47 phox in 32 P i-
loaded Epstein-Barr virus-transformed B lymphoblasts expressing wild type p47 phox or any …