Improved method for quantification of tissue PMN accumulation measured by myeloperoxidase activity
C Schierwagen, AC Bylund-Fellenius… - Journal of …, 1990 - Elsevier
Myeloperoxidase (MPO) was used as a marker enzyme for measuring polymorphonuclear
leukocyte (PMN) accumulation in tissue samples. The MPO recovery from kidney, liver,
myocardium, skeletal muscle (iliopsoas), and skin was measured, and a variation of 5%–
100% was found, depending on the tissue analyzed. The recovery could be improved to
100% in all tissues by incubation of the tissue samples at 60° C for 2 hr. This improved
method was used to measure PMN accumulation in rabbit myocardium after regional …
leukocyte (PMN) accumulation in tissue samples. The MPO recovery from kidney, liver,
myocardium, skeletal muscle (iliopsoas), and skin was measured, and a variation of 5%–
100% was found, depending on the tissue analyzed. The recovery could be improved to
100% in all tissues by incubation of the tissue samples at 60° C for 2 hr. This improved
method was used to measure PMN accumulation in rabbit myocardium after regional …