“PERFEXT”: a direct method for quantitative assessment of cytokine production in vivo at the local level
M Villavedra, H Carol, M Hjulström, J Holmgren… - Research in …, 1997 - Elsevier
A method termed “PERFEXT”, based on sequential perfusion and detergent extraction of
lymphoid and non-lymphoid organs, has been developed for the quantitative measurement
of cytokines produced at a local level in a given tissue. In vivo treatment of mice with
Staphylococcus enterotoxin B (SEB) or lipopoly-saccharide (LPS) served as the model
systems. Interleukin-2 (IL2) and interferon-γ (IFNγ) levels were monitored by ELISA analysis
of extracted samples. After local footpad (FP) injection with SEB, spleen and serum IL2 …
lymphoid and non-lymphoid organs, has been developed for the quantitative measurement
of cytokines produced at a local level in a given tissue. In vivo treatment of mice with
Staphylococcus enterotoxin B (SEB) or lipopoly-saccharide (LPS) served as the model
systems. Interleukin-2 (IL2) and interferon-γ (IFNγ) levels were monitored by ELISA analysis
of extracted samples. After local footpad (FP) injection with SEB, spleen and serum IL2 …