[HTML][HTML] Insulin-stimulated activation of eNOS is independent of Ca2+ but requires phosphorylation by Akt at Ser1179
M Montagnani, H Chen, VA Barr, MJ Quon - Journal of Biological Chemistry, 2001 - ASBMB
Vasodilator actions of insulin are mediated by activation of endothelial nitric-oxide synthase
(eNOS) and subsequent production of NO. Phosphatidylinositol 3-kinase and Akt play
important roles in insulin-signaling pathways leading to production of NO in vascular
endothelium. Here we dissected mechanisms whereby insulin activates eNOS by using the
fluorescent dye DAF-2 to directly measure NO production in single cells. Insulin caused a
rapid increase in intracellular NO in NIH-3T3 IR cells transiently transfected with eNOS. The …
(eNOS) and subsequent production of NO. Phosphatidylinositol 3-kinase and Akt play
important roles in insulin-signaling pathways leading to production of NO in vascular
endothelium. Here we dissected mechanisms whereby insulin activates eNOS by using the
fluorescent dye DAF-2 to directly measure NO production in single cells. Insulin caused a
rapid increase in intracellular NO in NIH-3T3 IR cells transiently transfected with eNOS. The …