[HTML][HTML] Role of phosphoinositide 3-kinase in monocyte recruitment under flow conditions
RE Gerszten, EB Friedrich, T Matsui, RR Hung… - Journal of Biological …, 2001 - ASBMB
Chemokines such as the monocyte chemol attractant protein-1 (MCP-1) convert monocyte
rolling to firm arrest under physiological flow conditions via integrin activation and
simultaneously activate phosphoinositide 3-kinase (PI3K). Here we used adenoviral gene
transfer and biochemical inhibitors to manipulate PI3K-dependent pathways in human
monocytes. In in vitro lipid kinase assays from purified human monocytes, we showed that
MCP-1 activates the" classical" PI3Kα pathway and not PI3Kγ, a PI3K isoform thought to be …
rolling to firm arrest under physiological flow conditions via integrin activation and
simultaneously activate phosphoinositide 3-kinase (PI3K). Here we used adenoviral gene
transfer and biochemical inhibitors to manipulate PI3K-dependent pathways in human
monocytes. In in vitro lipid kinase assays from purified human monocytes, we showed that
MCP-1 activates the" classical" PI3Kα pathway and not PI3Kγ, a PI3K isoform thought to be …