Corticotrophin-releasing factor-like immunoreactivity and mRNA in human leukocytes

A Stephanou, DS Jessop, RA Knight… - Brain, behavior, and …, 1990 - Elsevier
A Stephanou, DS Jessop, RA Knight, SL Lightman
Brain, behavior, and immunity, 1990Elsevier
Unstimulated normal leukocytes were studied for their expression of corticotrophin-releasing
factor (CRF)-like mRNA and peptide. In situ hybridization demonstrated CRF mRNA in T and
B lymphocytes and in neutrophils. Northern blot analysis also detected the presence of a
CRF mRNA species of approximately 1.7 Kb from lymphocytes and a peptide that reacted
with anti-CRF antiserum was also detected by radioimmunoassay. This lymphocyte-derived
peptide eluted earlier than standard CRF on high performance liquid chromatography …
Abstract
Unstimulated normal leukocytes were studied for their expression of corticotrophin-releasing factor (CRF)-like mRNA and peptide. In situ hybridization demonstrated CRF mRNA in T and B lymphocytes and in neutrophils. Northern blot analysis also detected the presence of a CRF mRNA species of approximately 1.7 Kb from lymphocytes and a peptide that reacted with anti-CRF antiserum was also detected by radioimmunoassay. This lymphocyte-derived peptide eluted earlier than standard CRF on high performance liquid chromatography. These findings suggest that regulatory mechanisms within the hypothalamus and the immune system may utilize closely related peptides.
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