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History Title: Competitive PCR. Language: English Authors: Siebert, Paul Larrick, James W.
Source: Nature Document Type: Article Abstract Informs that competitive reverse
transcription-polymerase chain reaction (RT-PCR) can be used to obtain quantitative information …

PCR has proved useful in amplifying specific mRNAs, especially those present in low copy
number. For example, it has been possible to amplify, subclone, and characterize low-
abundance mRNA (Prohman et a1. 1988] and to detect unique mRNA transcripts from
abnormal cells in a background of normal cells (Chelly et a1. 1988; Kawasaki et a]. 1988,
Lee et a1. 1987). Although it has been possible to detect and amplify large amounts of rare
mRNA transcripts, it has been more difficult to quantitate the amount of mRNA present in the …