Structure of tumour necrosis factor by X-ray solution scattering and preliminary studies by single crystal X-ray diffraction
A Lewit-Bentley, R Fourme, R Kahn, T Prangé… - Journal of molecular …, 1988 - Elsevier
A Lewit-Bentley, R Fourme, R Kahn, T Prangé, P Vachette, J Tavernier, G Hauquier, W Fiers
Journal of molecular biology, 1988•ElsevierThe structure of tumour necrosis factor has been investigated by X-ray small-angle
scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is
25· 5 Å. A plausible model accounting for the scattering curves consists of an elongated
trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 Å. Tumour
necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a
single trimer in the asymmetric unit. The diffraction extends to 3· 5 Å.
scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is
25· 5 Å. A plausible model accounting for the scattering curves consists of an elongated
trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 Å. Tumour
necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a
single trimer in the asymmetric unit. The diffraction extends to 3· 5 Å.
Abstract
The structure of tumour necrosis factor has been investigated by X-ray small-angle scattering and X-ray diffraction using synchrotron radiation. The overall radius of gyration is 25·5 Å. A plausible model accounting for the scattering curves consists of an elongated trimer with an axial ratio of 3 to 4 and a maximal chord with a lower limit of 80 Å. Tumour necrosis factor has been crystallized in a trigonal space group. Our results are in favour of a single trimer in the asymmetric unit. The diffraction extends to 3·5 Å.
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