[HTML][HTML] Dissociation of the high density lipoprotein and low density lipoprotein binding activities of murine scavenger receptor class B type I (mSR-BI) using retrovirus …
X Gu, R Lawrence, M Krieger - Journal of biological chemistry, 2000 - ASBMB
The murine class B, type I scavenger receptor (mSR-BI) is a receptor for both high density
lipoprotein (HDL) and low density lipoprotein (LDL) and mediates selective, rather than
endocytic, uptake of lipoprotein lipid. We have developed a" retrovirus library-based activity
dissection" method to generate mSR-BI mutants in which some, but not all, of the activities of
this multifunctional protein have been disrupted. This method employs three techniques: 1)
efficient in vitro cDNA mutagenesis (here error-prone PCR was used), 2) efficient retroviral …
lipoprotein (HDL) and low density lipoprotein (LDL) and mediates selective, rather than
endocytic, uptake of lipoprotein lipid. We have developed a" retrovirus library-based activity
dissection" method to generate mSR-BI mutants in which some, but not all, of the activities of
this multifunctional protein have been disrupted. This method employs three techniques: 1)
efficient in vitro cDNA mutagenesis (here error-prone PCR was used), 2) efficient retroviral …