T-cell antigen receptor Vβ subsets are not preferentially deleted in AIDS
DN Posnett, S Kabak, AS Hodtsev, EA Goldberg… - Aids, 1993 - journals.lww.com
DN Posnett, S Kabak, AS Hodtsev, EA Goldberg, A Asch
Aids, 1993•journals.lww.comObjective: Evidence for an HIV-1-associated superantigen (SAg) has been presented. Here
we test whether targeted T cells are preferentially depleted in AIDS. Design: The T cell
antigen receptor (TCR) VP repertoire was compared in 44 HIV+ hemophiliac patients and in
27 controls. Methods: Eleven TCR V gene-specific monoclonal antibodies (MAb),
representing approximately one-third of the VP repertoire were used to measure T-cell
numbers expressing V [beta] and V [alpha] genes in both the CD4 and the CD8 subsets by …
we test whether targeted T cells are preferentially depleted in AIDS. Design: The T cell
antigen receptor (TCR) VP repertoire was compared in 44 HIV+ hemophiliac patients and in
27 controls. Methods: Eleven TCR V gene-specific monoclonal antibodies (MAb),
representing approximately one-third of the VP repertoire were used to measure T-cell
numbers expressing V [beta] and V [alpha] genes in both the CD4 and the CD8 subsets by …
Abstract
Objective: Evidence for an HIV-1-associated superantigen (SAg) has been presented. Here we test whether targeted T cells are preferentially depleted in AIDS.
Design: The T cell antigen receptor (TCR) VP repertoire was compared in 44 HIV+ hemophiliac patients and in 27 controls.
Methods: Eleven TCR V gene-specific monoclonal antibodies (MAb), representing approximately one-third of the VP repertoire were used to measure T-cell numbers expressing V [beta] and V [alpha] genes in both the CD4 and the CD8 subsets by two-color immunofluorescence.
Results: The percentage of T cells expressing these V genes could be accurately quantitated in both the CD4 and the CD8 T-cell populations and at various clinical stages of disease progression. No preferential deletion of any particular V gene subsets was detected.
Conclusion: CD4 T-cell depletion in AIDS is not V [beta]-selective.
(C) Lippincott-Raven Publishers.
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